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The molecular weight was determined by Tricine - SDS - PAGE .

Tricine-SDS-PAGE电泳检测其相对分子质量。

The Tricine SDS-PAGE revealed 8 belts of protein .

tricineSDS-PAGE电泳显示8条蛋白带；

After induction , a specific band could be detected in the extracts of the host strain by Tricine SDS PAGE and Western blotting .

利用TricineSDSPAGE和WESTERNBLOTTING均可在诱导后的宿主菌中检测到特异蛋白带。

With RSM the optimum values of the optimum buffer composition were obtained as follows : 12 mmol / L sodium chloride , 12 mmol / L tricine , 12 mmol / L sodium acetate , 3.0 mmol / L putrescine ;

数学分析得到的缓冲液组分为：12mmol/L氯化钠，12mmol/L麦黄酮，12mmol/L醋酸钠，3.0mmol/L腐胺；